e-mail: silvia.jimeno@cabimer.es
‘Ramón y Cajal’ Researcher, Universidad de Sevilla, Spain
Research line:
Regulation of transcription elongation and pre-mRNA processing by chromatin and DNA topology
e-mail: silvia.jimeno@cabimer.es
‘Ramón y Cajal’ Researcher, Universidad de Sevilla, Spain
Research line:
Regulation of transcription elongation and pre-mRNA processing by chromatin and DNA topology
During transcription elongation, the RNA polymerase II (RNAPII) has to overcome obstacles such as the presence of nucleosomes or the topological stress produced by the movement of the transcription complex. My work has been focused on how RNAPII coordinates transcription with the removal of those obstacles. I am particularly interested in studying whether the pauses that are associated to nucleosome remodeling or supercoiling removal could serve as a mechanism for the coupling of transcription elongation with pre-mRNA processing.
We have discovered that positioning of the first (+1) nucleosome in a gene participates in the regulation of promoter-proximal pausing, a highly-regulated pause that takes place in early elongation. Interestingly, in the absence of such nucleosome pre-mRNA capping is affected (Jimeno-González, 2015#1). Furthermore, disrupting chromatin structure globally by histone depletion accelerates transcription rate and, as a consequence, splicing defects are observed (Jimeno-González et al, 2015#2). From a different point of view, I have been focused on how changing DNA supercoiling levels by topoisomerase II inhibition alters transcription regulation obtaining results that point to a deep relevance of DNA topology in the control of different transcriptional steps.
Previous positions:
ORCID: 0000-0002-6223-9573
Researcher ID: C-2207-2017
Scopus: 15132366300