Centro Andaluz de Biología Molecular y Medicina Regenerativa
Tatiana García-Muse

e-mail: tatiana.muse@cabimer.es

 

Main Research Lines: 

  1. Understanding the impact of DNA damage-dependent phosphorylations to maintain genome stability in meiotic cells.
  1. Transcription-associated genome instability: R-loops and chromatin condensation using C. elegans as model system.

Upon graduating in Biology at the University of Seville in 1998, I joined Dr. Pérez-Martín laboratory at Centro Nacional de Biotecnología (Madrid). There I succesfully defended my Thesis in 2003. I was the first person to join Dr. Pérez-Martín laboratory, hence we had to stablish all the basic know-how from the beginning. Our work highlighted the relevance of cell cycle regulation during the pathogenesis and helped to define the connections between cell cycle and the pathogenesis program induction of U. maydis (Eukaryot Cell 2003, J. Cell Sci. 2004 and J. Cell Sci. 2004).

During my thesis period I developed a keen interest in understanding how cell cycle progressing is controlled by DNA damage checkpoints, also I wanted to explore an alternative model system. For these reason I chose to do a postdoc in Dr. Boulton’s group at Clare Hall laboratories-CRUK (London), which uses Caenorhabditis elegans as a model system to understand the DNA damage response. During this period my research contributed to understand how the DNA damage response (DDR) senses DNA lessions and how triggers the activation of DNA repair pathways during meiosis and mitosis to repair such lessions (EMBO J. 2005, EMBO J. 2006 and EMBO Rep. 2006).

In February 2008, with a JAE-doc contract, I joined Dr. Aguilera´s group at CABIMER (Sevilla), to continue my research in DDR and to expand my expertise in the mechanisms that lead to genomic instability. I have been in his laboratory ever since as a Senior Researcher leading C. elegans research lines that complement the interest of Dr. Aguilera regarding the effects of transcription in genome stability in metazoans. My duties include the co-supervision of PhD students. This period has led so far to three research publications in Mol. Cell, 2013, EMBO Rep** 2012 and PLosOne* 2013 (correspondig author* or co-corresponding**); as well as several review articles.

Additionally, I have continued my own project uncovering the in vivo relevance of a DNA damage-dependent phosphorylation identified by peptide array at the end of my stay of Boulton’s laboratory, which has led to a manuscrip published in Cell Rep.** 2019 (co-corresponding**) which shows how the phosphorylation of the synaptonemal complex, a protein structure that forms between homologous chromosomes during meiosis, bias the repair of persistent DSBs towards inter-sister recombination. Importantly this work validates the data I obtained in the peptide array screening that is the base of my research to undertand DNA damage during meiosis.

My long term goal is to address at the molecular level the biological relevance of the DNA damage-dependent phosphorylations at meiotic proteins to ensure genome stability. Understanding this is of vital importance in order to have a major comprehensive view of the sources of errors that result in dramatically deleterious outcomes including infertility, miscarriages and birth defects such as Down syndrome. This knowledge of DDR regulation during meiosis should, therefore, provide important insights into fertility defects diagnosis and may present opportunities for therapeutic intervention.

ORCID: 0000-0002-3195-2261
ResearcherID: F-1339-2016
Scopus: 6506810877 

Genomic DNA is under constant attack from both endogenous and exogenous DNA damaging agents. Without proper repair the resulting DNA damages would lead to alteration of genomic structure thus affecting the faithful transmission of genetic information. In addition, defects during meiosis lead to aneuploidy, an extreme kind of genetic instability associated with fertility problems, miscarriages and mental retardation syndromes. Very little is known about the relationship between the meiosis progression and the DNA damage response. Since cells undergoing meiosis during oogenesis stay arrested in meiosis I for long periods of time and therefore vulnerable to DNA lesions we speculated if the increase in genome instability inferred from the increase in aneuploidy that correlates with mother age might be related to defects in DDR during meiosis. Phosphorylation is a key regulator in many processes and therefore an obvious first candidate to analyse, and key kinases of DNA damage checkpoints are ATR and ATM. The aim is to address at the molecular level the biological relevance of the DNA damage dependent phosphorylations observed in silico with the peptide array technique and to determine how ATR/ATM DNA damage phosphorylations contribute to the regulation of different DNA repair pathways, both to ensure genome stability of meiotic cells. Regarding genome stability I am also involved in research lines which objective are determine the mechanisms by which cells solve transcription-replication conflicts to prevent genome instability with emphasis in RNA-DNA hybrids and its effects in chromatin condensation.


Projects as Principal Investigator:

– Fosforilaciones de ATM/ATR en Respuesta al Daño en el ADN durante meiosis (PGC2018-101099-B-I00). Ministerio de Ciencia, Innovación y Universidades.
– Fosforilaciones de ATM/ATR en Respuesta al Daño en el ADN (2018/00000498) Ayudas a proyectos precompetitivos Plan Propio US.
– Fosforilaciones de ATM/ATR en Respuesta al Daño en el ADN durante meiosis (2019/00000463). Ayuda por captación de fondos en las convocatorias de proyectos de investigación del plan estatal Plan Propio US.

Projects as Researcher:

– Transcription y Replication Como Fuentes de Inestabilidad Genética (BFU2016-75058-P). Ministerio de Economía, Industria y Competitividad.
– R-loops as a major modulator of transcription-associatedrecombination and chromatin dynamics (TARLOOP. ERC Advanced Grant). European Research Council.

Articles:

García-Muse T*, Galindo U, Garcia-Rubio M, Martin JS, Polanowska J, O’Reilly N, Aguilera A*, Boulton SJ*. 2019. A meiotic checkpoint alters repair partner bias to permit inter-sister repair of persistent DSBs. (*co-corresponding). Cell Rep. 26: 775-787

Castellano-Pozo M, Santos-Pereira, JM, Rondón, AG, Barroso, S, Andújar, E, Pérez-Alegre M, Garcia-Muse T and Aguilera A. 2013. R-loops are linked to histone H3 Ser10 phosphorylation and chromatin condensation. Mol. Cell 52: 583-590.

Castellano-Pozo M, Garcia-Muse T* and Aguilera A. 2012. The Caenorhabditis elegans THO complex is required for the mitotic cell cycle and development. PLoS One 7: e52447. (*corresponding).

Castellano-Pozo M , Garcia-Muse T* and Aguilera A*. 2012. R-loops cause replication impairment and genome instability during meiosis. EMBO Rep. 13: 923-9. (*co-corresponding).

Dengg M*, Garcia-Muse T*, Salus SS, Gill SS, Ashcroft N, Boulton SJ, and Nilsen H.  2006. Abrogation of the CLK-2/RAD-5 checkpoint leads to tolerance to persistent repair intermediates generated by base-excision repair. EMBO Rep. 10: 1046-1051. (*Joint first).

Polanowska J, Martin JS, Garcia-Muse T, Petalcorin, MIR, Boulton, SJ. 2006. A conserved pathway to activate BRCA1-mediated ubiquitylation at DNA damage sites. EMBO Journal 25: 2178-2188.

García-Muse T and Boulton SJ. 2005. Distinct modes of ATR activation after replication stress and DNA double-strand breaks in Caenorhabditis elegans. EMBO J. 24: 4345-4355.

Castillo-Lluva S*, García-Muse T*, Pérez-Martín J. 2004. A member of the Fizzy-related family of APC activators is regulated by cAMP and is required at different stages of plant infection by Ustilago maydis. J Cell Sci. 117: 4143-56. (*Joint first)

García-Muse T, Steinberg G, Pérez-Martín, J. 2004. Characterization of B-type cyclins in the smut fungus Ustilago maydis: roles in morphogenesis and pathogenicity. J Cell Sci. 117(Pt 3): 487-506.

García-Muse T, Steinberg G, Pérez-Martín J. 2003. Pheromone-induced G2 arrest in the phytopathogenic fungus Ustilago maydis. Euk. Cell 2(3): 494-500.

Reviews:

García-Muse T, Aguilera A. 2016. Transcription-replication conflicts: how they occur and how they are resolved. Nat Rev Mol Cell Biol. 17(9): 553-63.

Gaillard H, García-Muse T, Aguilera A. 2015. Replication stress and cancer. Nat Rev Cancer 15(5): 276-89.

Aguilera A and García-Muse T. 2013. Causes of genome instability. Annu. Rev. Genet. 47: 1-32.

Aguilera A and García-Muse T. 2012. R-loops: form transcription by-products to threats to genome stability. Mol. Cell 46: 115-24.

Garcia-Muse T, Boulton SJ. 2007. Meiotic recombination in Caenorhabditis elegans. Chromosome Res. 15(5): 607-21.